Fig. 1. The effect of IVM on RAW264.7 cells. Cell viability inhibition of RAW264.7 cells treated with various concentrations of IVM (1.25, 2.5, 5,10,15 and 20 μM) for 24 h and 48 h. Small alphabets indicate significant differences (p < 0.05) between any two groups (A). Data are shown as the means ± SD of triplicate measurements.
ソース
Immunotoxicity induced by Ivermectin is associated with NF-κB signaling pathway on macrophages
The results revealed that IVM increased the frequency of epithelial tumor in D. melanogaster considering all evaluated concentrations, while AMX showed no carcinogenic effect.
Our current observation allows us to communicate for the first time the in vitro evaluation of DNA damage produced by these antiparasiticides: a brief 80 min pulse-treatment of 5.0–50.0 μg/ml of IVM or 25.0 and 50.0 μg/ml of ivomec®, resulted in a manifest level of single DNA-strand break induction. This findings are in concordance with previous observations showing a genotoxic effect exerted by the abamectin, the most active form of the avermectin (avermectin B1a), able to induce single strand DNA breaks in rat hepatocytes from rats treated in vivo [27].
IVM reduced testicular volume, tubular diameter and germinal epithelium height. Spermatogenesis interruption, such as degeneration morphology of the germ cells